叶黄素对母鼠乳腺分泌IgA的促进作用

为明确叶黄素对母鼠乳腺分泌IgA能力的影响，本实验对妊娠母鼠添加饲喂叶黄素，检测母鼠血清中IgG、IgA和IgM含量变化，母鼠乳腺组织中IgA mRNA表达量和IgA抗体分泌细胞数量变化，以及仔鼠胃内容物中IgA浓度变化。结果表明：在妊娠21 d和分娩后7 d时，实验组血清中IgG和IgA浓度显著高于正常对照组（P＜0.05），IgM含量虽有升高，与正常对照组相比，无显著性差异（P＞0.05）；乳腺组织中IgA mRNA表达量无显著变化（P＞0.05）；分娩后14 d IgA抗体分泌细胞数量显著增加（P＜0.05）；仔鼠胃内容物中IgA含量极显著高于对照组（P＜0.01）。说明给妊娠母鼠添加饲喂叶黄素可以增强乳腺分泌IgA的能力。

Promoting Effect of Lutein on the IgA Secretion Capacity of Mammary Glands in Maternal Mice

The present experiment was conducted to explore the effect of supplemental lutein on the serum concentrations of
IgG, IgA and IgM, the mRNA expression of IgA C-region and the number of IgA antibody-secreting cells (ASC) in breast
of maternal mice as well as IgA concentration in stomach contents of neonatal mice. Maternal mice were fed rodent feed or
50 mg/kg lutein-supplemented rodent feed during pregnancy and after parturition. The serum concentrations of IgG and
IgA increased significantly at 21 days of pregnancy and at 7 days after parturition (P < 0.05), but IgM concentration and the
mRNA expression of IgA C-region were not significantly affected by lutein supplementation (P > 0.05). The number
of IgA ASC at 14 days after parturition (P < 0.05) and the IgA concentration in stomach contents of neonatal mice at 7 and
14 days after parturition were enhanced markedly (P < 0.01) in response to lutein supplemention. These results indicated that
supplemental lutein for maternal mice is effective in enhancing the secretion of IgA in mammary glands.