蒸汽爆破预处理对粉葛总黄酮及抗氧化性的影响

实验选取爆破压力1.0、2.0 MPa,维持压力时间30、40、60、80 s,对粉葛进行预处理。利用紫外分光光度法表征爆破前后总黄酮的变化,旨在探讨蒸汽爆破预处理对粉葛总黄酮及抗氧化性的影响。抗氧化活性利用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)清除率进行评价。结果表明:经过蒸汽爆破预处理后,粉葛的总黄酮提取量、甲醇提取物得率和抗氧化性得到显著提高。在最优处理条件下,总黄酮(以葛根素计)提取量达到5.43 mg/g,是未经预处理粉葛提取量的2.32倍;甲醇提取物得率为16.92%;抗氧化活性得到显著增加,DPPH清除活性的半数抑制浓度(50%inhibitory concentration,IC50)由30.65 g/L降低至10.10 g/L。将蒸汽爆破预处理应用于粉葛活性物质的提取,可实现提取量和抗氧化能力的有效提高。

Effect of Steam Explosion on Total Isoflavone Content and Antioxidant Capacity of Puerariae thomsonii Radix

Steam explosion pretreatment was employed on Puerariae thomsonii Radix at a steam pressure of 1.0 and
2.0 MPa for 30, 40, 60 and 80 s, respectively. In order to explore the effect of steam explosion pretreatment on the total
isoflavone content and antioxidant capacity of Puerariae thomsonii Radix, the content of total isoflavones was determined
using UV spectrophotometry. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used to evaluate
free radical scavenging capacity. The results indicated that the extraction efficiency of total isoflavones, the yield of
methanol extract and antioxidant capacity significantly increased after steam explosion. Under optimal conditions, the
yield of total isoflavones (calculated as puerarin) was 5.43 mg/g, which was 2.32 times as much as that of the untreated
sample; the yield of methanol extract was 16.92%, and the 50% inhibitory concentration (IC50) for DPPH radical scavenging
activity decreased from 30.65 g/L to 10.10 g/L, suggesting that the antioxidant capacity was improved. It can be concluded
that a proper and reasonable steam explosion pretreatment can be applied to extract the active compounds and enhance the
antioxidant capacity of Puerariae thomsonii Radix.