| 气相色谱法同时测定食品中的胆固醇和植物甾醇 |
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| 引用本文： | 陈月晓,何 涛,唐凌轩,徐佳佳,白沙沙,崔亚娟,何 梅,李 东,黄 华,路 勇.气相色谱法同时测定食品中的胆固醇和植物甾醇[J].食品科学,2016,37(14):180-183. |
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| 作者姓名： | 陈月晓 何 涛 唐凌轩 徐佳佳 白沙沙 崔亚娟 何 梅 李 东 黄 华 路 勇 |
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| 作者单位： | 1.北京市营养源研究所，北京 100069；2.北京市食品安全监控和风险评估中心，北京 100041 |
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| 基金项目： | 首都居民膳食结构研究与数据平台搭建项目（Z141100002614013）；创新工程Ⅱ-3项目：食物中四类植物化学物
检测技术的开发改进及植物化学物配方食品加工方法研究项目（PXM2014-178102-000011） |
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| 摘 要： | 建立气相色谱法同时测定食品中植物甾醇和胆固醇含量的分析方法，测定植物甾醇含量较高的常见植物油和同时含有胆固醇及植物甾醇的样品。采用5α-胆甾烷为内标参照物，样品经皂化后提取，浓缩后正庚烷定容上机进样分析。胆固醇和植物甾醇在0.001～2.5 mg/mL质量浓度范围内线性良好，线性相关系数R2均大于0.99。方法的检出限为0.3 mg/100 g，定量限为1.0 mg/100 g，该方法相较于液相色谱法能有效将胆固醇和植物甾醇进行分离，具有步骤简单、重复性好、准确度和灵敏度高等特点。
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| 关 键 词： | 气相色谱法 胆固醇 植物甾醇 |
Simultaneous Determination of Cholesterol and Phytosterol in Foods by Gas Chromatography |
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| CHEN Yuexiao,HE Tao,TANG Lingxuan,XU Jiajia,BAI Shasha,CUI Yajuan,HE Mei,LI Dong,HUANG Hua,LU Yong.Simultaneous Determination of Cholesterol and Phytosterol in Foods by Gas Chromatography[J].Food Science,2016,37(14):180-183. |
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| Authors: | CHEN Yuexiao HE Tao TANG Lingxuan XU Jiajia BAI Shasha CUI Yajuan HE Mei LI Dong HUANG Hua LU Yong |
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| Affiliation: | 1. Beijing Research Institute for Nutritional Resources, Beijing 100069, China;
2. Beijing Municipal Center for Food Safety Monitoring and Risk Assessment, Beijing 100041, China |
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| Abstract: | A method for the simultaneous determination of phytosterol and cholesterol in foods was established by gas
chromatography (GC) in this research. Cholesterol, brassicasterol, campesterol, stigmasterol, β-sitosterol, and stigmasterol
were quantified using 5α-cholestane as the internal reference standard. Samples were saponified before extraction and the
extract was concentrated and diluted again with n-heptane before being injected into the chromatographic system. The results
showed that cholesterol and phytosterols were separated well and displayed linear relationship over the concentration range
of 0.001–2.5 mg/mL. The limit of detection (LOD) and limit of quantitation (LOQ) of this method were 0.3 and 1.0 mg/100 g,
respectively. Compared with high performance liquid chromatography (HPLC), the GC method could effectively separate
cholesterol and phytosterols, with simpler operation, better reproducibility, and higher accuracy and sensitivity. |
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| Keywords: | gas chromatography cholesterol phytosterol |
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