海参内脏酶解产物抗氧化和抗疲劳活性

以海参内脏为原料,通过中性蛋白酶水解,结合超滤和冷冻干燥工艺制备海参内脏酶解产物,利用高效液相色谱法测定酶解产物的分子质量。结果表明,海参内脏酶解产物中蛋白质含量为73.02%,以分子质量分布在187~998 D的小分子肽为主,该部分峰面积占总面积的77.9%。酶解产物具有一定的抗氧化活性,不同剂量(200、400、800 mg/(kg·d))的酶解产物均能降低小鼠肝脏的丙二醛含量,增加超氧化物歧化酶活力和还原型谷胱甘肽含量,且与模型对照组相比,差异均达到极显著水平(P0.01),说明海参内脏酶解产物能有效恢复氧化损伤机体抗氧化指标。海参内脏酶解产物具有一定的抗疲劳功效,3个剂量组均能极显著延长小鼠的力竭游泳时间和提高运动小鼠的肝糖原含量,降低其血清乳酸和血清尿素氮含量(P0.01)。

Antioxidant and Antifatigue Activities of Enzymatic Protein Hydrolysate from Sea Cucumber Viscera

Protein hydrolysate from visceral waste proteins of sea cucumber was prepared by hydrolysis with neutral protease, followed by ultrafiltration and freeze drying. The protein content in the protein hydrolysate was 73.02%, which was composed mainly of small peptides. The results of high performance liquid chromatography analysis showed that the relative molecular weight of the hydrolysate was mainly distributed in the range of 187–998 D, accounting for 77.9% of the total peak area. In vitro antioxidant experiments showed that the protein hydrolysate had significant antioxidant activity. The hydrolysate at various dosages (200, 400 and 800 mg/(kg·d)) could significantly reduce the malonaldehyde content in mouse livers, and also increase the activity of superoxide dismutase, and glutathione contents (P < 0.01). The results illustrated that the hydrolysate could effectively restore antioxidant indexes. The experiments of antifatigue activity proved that the hydrolysate had high antifatigue activity. All three dosages of the hydrolysate could significantly increase the exhaustive swimming time and liver glycogen contents of mice, and reduce the contents of lactic acid and urea nitrogen in the serum of mice (P < 0.01).