紫色杆菌素及脱氧紫色杆菌素的基因毒性评价

目的：考察紫色杆菌素、脱氧紫色杆菌素以及二者混合物的基因毒性，为其更广泛的活性研究和应用奠定基础。方法：利用SOS/umu检测试剂盒定量分析紫色杆菌素、脱氧紫色杆菌素、二者混合物以及各自的S9代谢产物是否刺激鼠伤寒沙门菌Salmonella typhimurium NM2009细胞产生了SOS修复，以及与阳性药物2-氨基芴（2-aminofluorene，AF-2）和2-氨基蒽（2-aminoanthracene，2-AA）进行对比所产生毒性作用的强弱。结果：32.6 μg/mL的脱氧紫色杆菌素、经S9代谢后的34.2 μg/mL的紫色杆菌素和33.4 μg/mL的混合物对S. typhimurium NM2009细胞产生了基因毒性，但与阳性对照物相比属于低毒性物质，且相同剂量的紫色杆菌素和脱氧紫色杆菌素以及二者混合物的基因毒性基本相同，二者混合后未产生协同效应。结论：紫色杆菌素和脱氧紫色杆菌素属于低基因毒性物质。

Genotoxicity Evaluation of Violacein and Deoxyviolacein by umu Test

Objective: Violacein and deoxyviolacein are bacteria-originated indole compounds with diverse bioactivities.
This study aimed to investigate the genotoxicity of violacein, deoxyviolacein and their combination, and provide the basis
for the exploration of their applications. Methods: The SOS repairing of Salmonella typhimurium NM2009 cells induced
by violacein, deoxyviolacein, their combination or S9 metabolites was quantitatively analyzed by SOS/umu detection kits.
Meanwhile, the toxicity of violacein, deoxyviolacein, their combination and S9 metabolites as well as the positive control
drugs such as 2-aminofluorene (AF-2) and 2-aminoanthracene (2-AA) was compared. Results: It was demonstrated that
32.6 μg/mL deoxyviolacein, S9 metabolites of 34.2 μg/mL violacein and 33.4 μg/mL combination had genotoxicity for
Salmonella typhimurium NM2009 cells, but they all showed lower genotoxicity compared with positive control. Moreover,
violacein, deoxyviolacein and their combination at the same dosage exhibited similar toxicity without synergistic effect.
Conclusion: Violacein and deoxyviolacein have weak genotoxicity.