高效液相色谱法同时测定葡萄酒中16 种添加剂

研究同时测定葡萄酒中16 种添加剂的高效液相色谱方法。该法主要采用C A P C E L L P A K C 18柱（150 mm×4.6 mm，5 μm）分离，以甲醇-20 mmol/L乙酸铵溶液为流动相，采取梯度洗脱，在波长250 nm处进行测定。结果表明，在0.1～20 mg/L的质量浓度范围内线性相关系数良好，相关系数范围为0.999 3～0.999 7，方法检出限范围为0.01～0.5 mg/L，相对标准偏差在0.3%～6.3%之间，平均回收率范围在76.5%～108.5%之间。本方法简便、可靠，能满足对市场上葡萄酒中相关非法添加剂的检测监控要求。

Simultaneous Determination of 16 Additives in Wine by High Performance Liquid Chromatography

A high performance liquid chromatography (HPLC) method was developed in this study for the simultaneous
determination of 16 additives in wine. Chromatographic separation was performed using gradient elution on CAPCELL PAK
C18 column (150 mm × 4.6 mm, 5 μm) with methanol-20 mmol/L ammonium acetate as mobile phase, followed by detection at
250 nm. The results showed that the method exhibited an excellent linearity in the concentration range of 0.1–20 mg/L with a
correlation coefficient of 0.999 3–0.999 7 and had a good repeatability with relative standard deviation (RSD) of 0.3%–6.3%. The
limits of detection (LOD) were between 0.01 and 0.5 mg/L, and average recoveries ranged from 76.5% to 108.5%. The present
method is simple and accurate and can meet the requirements for the analysis of illicit additives in wines.