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贵州地区“紫红龙”火龙果采后病原菌分离鉴定
引用本文:李国林,钱其军,孟繁博,陈曦,黄道梅,郑秀艳,林茂.贵州地区“紫红龙”火龙果采后病原菌分离鉴定[J].食品安全质量检测技术,2018,9(21):5647-5652.
作者姓名:李国林  钱其军  孟繁博  陈曦  黄道梅  郑秀艳  林茂
作者单位:贵州省农业科学院现代农业发展研究所,仁怀市后山乡农业中心,贵州省农业科学院现代农业发展研究所,贵州省农业科学院现代农业发展研究所,贵州省农业科学院现代农业发展研究所,贵州省农业科学院现代农业发展研究所,贵州省农业科学院现代农业发展研究所
基金项目:黔农科院院专项[2016]026号、贵州省科技计划项目[2016]2535号、黔农科院自主创新科研专项字(2014)018号、贵州省特色农产品辐照保鲜技术服务企业行动计划[2016]5712、贵州省特色农产品辐照保鲜与加工工程技术研究中心(2016GZ67305)
摘    要:目的 分离鉴定贵州地区“紫红龙”火龙果在采后贮藏期间自然发病的病原菌。方法 “紫红龙”火龙果为试材, 通过涂布平板分离法以及划线纯化, 从贮藏期火龙果中分离、纯化采后病原菌, 并结合形态学观察与rDNA ITS (internal transcribed spacer)序列测定进行准确鉴定。结果 分离出1株真菌, 经鉴定该株病原菌为桃吉尔霉(Gilbertella persicaria)。结论 本研究在贵州地区腐烂的火龙果中首次分离得到G. persicaria, 该菌对红肉火龙果有较强的致病力。

关 键 词:“紫红龙”火龙果    采后病害    病原菌    桃吉尔霉
收稿时间:2018/9/4 0:00:00
修稿时间:2018/11/6 0:00:00

Isolation and identification of pathogen from Hylocereus undatus cv. Zihonglong in Guizhou province
LI Guo-Lin,QIAN Qi-Jun,MENG Fan-Bo,CHEN Xi,HUANG Dao-Mei,ZHENG Xiu-Yan and LIN Mao.Isolation and identification of pathogen from Hylocereus undatus cv. Zihonglong in Guizhou province[J].Food Safety and Quality Detection Technology,2018,9(21):5647-5652.
Authors:LI Guo-Lin  QIAN Qi-Jun  MENG Fan-Bo  CHEN Xi  HUANG Dao-Mei  ZHENG Xiu-Yan and LIN Mao
Affiliation:Integrated Agricultural Development Research Institute,Agricultural Service Center of Houshan village,Integrated Agricultural Development Research Institute,Integrated Agricultural Development Research Institute,Integrated Agricultural Development Research Institute,Integrated Agricultural Development Research Institute and Integrated Agricultural Development Research Institute
Abstract:Objective To isolate and identify the pathogenic bacteria of Hylocereus undatus cv. Zihonglong in Guizhou that naturally occurred during postharvest storage. Methods With Hylocereus undatus cv. Zihonglong as test material, the fungal were isolated and purified from dragon fruit during storage period by the spread plate and streak plate method, and it was accurately identified by the morphology studies and rDNA ITS (internal transcribed spacer) sequence analysis. Results One fungus was isolated, and the fungal was identified to be Gilbertella persicaria. Conclusion G. persicaria is first isolated from rotten dragon fruit in Guizhou province, and it has strong pathogenicity to red pitaya fruits.
Keywords:Hylocereus undatus cv  Zihonglong  postharvest disease  pathogen  Gilbertella persicaria
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