响应面试验优化“Amano”β-糖苷酶水解槐角异黄酮工艺

利用"Amano"β-糖苷酶对槐角异黄酮进行水解制备染料木黄酮。采用高效液相色谱法检测槐角异黄酮水解率。探讨酶质量浓度、水解时间、p H值和水解温度对异黄酮水解率的影响,并通过响应面法确定最佳水解条件。结果表明,最佳水解条件为酶质量浓度0.5 mg/m L、水解时间3.68 h、p H 4.95、水解温度59.2℃。在此条件下,槐角异黄酮水解率达95.76%。该研究为自然界药用植物的开发应用提供了理论依据。

Process Optimization by Response Surface Methodology for Hydrolysis of Fructus Sophorae Isoflavones by“Amano” β-Glucosidase

Purpose: To optimize the hydrolysis (into genistein) of isoflavones extracted from Fructus Sophorae with “Amano”
β-glucosidase. Methods: The optimization of four hydrolysis conditions including enzyme concentration, time, pH and
temperature for improved hydrolysis efficiency was performed using response surface methodology (RSM). Results: The
optimal hydrolysis conditions were determined as follows: enzyme concentration, 0.5 mg/mL; hydrolysis time, 3.68 h; pH
value, 4.95, and hydrolysis temperature, 59.2 ℃. Under these conditions, the hydrolysis efficiency of total isoflavones was as
high as 95.76%. Our investigation is significant for the exploitation and application of medicinal pants.