白酒窖泥中蛋白酶产生菌的筛选及复配

从优质白酒窖泥中分离筛选出8 株高产蛋白酶的兼性厌氧细菌，通过测定内肽酶、氨肽酶、羧肽酶酶活力，最终筛选出3 株3 种蛋白酶活力相对较高的菌株HDS4、HDS6、HDS8，并对其进行形态观察、生理生化和16S rDNA鉴定，确定3 株菌分别为枯草芽孢杆菌（Baci l lus subt i l i s）、解淀粉芽孢杆菌（Baci l lusamyloliquefaciens）、苏云金芽孢杆菌（Bacillus thuringiensis）。以接种量为基础，对3 株目的菌株进行复配，得到的复配菌系3 种蛋白酶活力均比单一菌株高，3 种酶活力分别提高了8.907%、6.181%、8.781%。

Screening of Protease-Producing Strains in Liquor Pit Mud and Mixed-Culture Fermentation for Enhanced Protease Production

Eight strains of facultative anaerobic bacteria with higher capacity to produce protease were isolated and screened
from high-quality liquor pit mud. Out of the 8 strains, 3 strains producing higher endopeptidase, aminopeptidase and
carboxypeptidase activities including HDS4, HDS6 and HDS8 were selected and identified by morphological, physiological
and biochemical properties and 16S rDNA sequence analysis as Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus
thuringiensis. Mixed-culture fermentation with the 3 strains yielded an increase of 8.907%, 6.181% and 8.781% in
endopeptidase, aminopeptidase and carboxypeptidase activities compared with single-culture fermentation, respectively.