| 大孔树脂纯化黑果腺肋花楸多酚的工艺优化 |
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| 引用本文： | 李 斌,高凝轩,刘 辉,孟宪军,张 琦,矫馨瑶,李恩惠,丑述睿,张 野,陈世富.大孔树脂纯化黑果腺肋花楸多酚的工艺优化[J].食品科学,2016,37(16):69-74. |
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| 作者姓名： | 李 斌 高凝轩 刘 辉 孟宪军 张 琦 矫馨瑶 李恩惠 丑述睿 张 野 陈世富 |
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| 作者单位： | 1.沈阳农业大学食品学院，辽宁 沈阳 110161；2.沈阳综合保税区出入境检验检疫局，辽宁 沈阳 110169；
3.辽宁富康源黑果腺肋花楸科技开发有限公司，辽宁 海城 114200 |
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| 基金项目： | 辽宁省高等学校优秀人才支持计划项目（2014108）；公益性行业（农业）科研专项（201303073-04）；
沈阳农业大学天柱山英才计划项目（2014） |
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| 摘 要： | 以黑果腺肋花楸为原料，采用大孔树脂纯化黑果腺肋花楸中多酚类物质。通过对比6 种大孔树脂对黑果腺肋花楸多酚吸附-解吸效果，筛选出XAD-7大孔树脂作为最佳纯化材料，并通过单因素试验确定XAD-7大孔树脂纯化黑果腺肋花楸多酚的静态吸附-解吸最佳工艺条件为：吸附时间4 h、解吸时间2 h、上样液质量浓度3.6 mg/mL、上样液pH 4、乙醇体积分数95%、乙醇溶液pH 7；其对黑果腺肋花楸多酚动态吸附-解吸最佳工艺条件为：上样流速2 mL/min、上样量560 mL、蒸馏水洗脱用量350 mL、洗脱流速2 mL/min、洗脱体积300 mL。在此条件下，黑果腺肋花楸多酚纯度由11.62%提高到64.37%，表明XAD-7大孔树脂对于黑果腺肋花楸多酚具有较好的纯化效果。
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| 关 键 词： | 黑果腺肋花楸 多酚 大孔树脂 纯化 |
Macroporous Adsorption Resin for the Purification of Polyphenols from Aronia melanocarpa Berries |
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| LI Bin,GAO Ningxuan,LIU Hui,MENG Xianjun,ZHANG Qi,JIAO Xinyao,LI Enhui,CHOU Shurui,ZHANG Ye,CHEN Shifu.Macroporous Adsorption Resin for the Purification of Polyphenols from Aronia melanocarpa Berries[J].Food Science,2016,37(16):69-74. |
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| Authors: | LI Bin GAO Ningxuan LIU Hui MENG Xianjun ZHANG Qi JIAO Xinyao LI Enhui CHOU Shurui ZHANG Ye CHEN Shifu |
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| Affiliation: | 1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China;
2. Shenyang Free Trade Zone Entry-Exit Inspection and Quarantine Bureau, Shenyang 110169, China;
3. Liaoning Fukangyuan Aroniamelanocarpa Technology Development Co. Ltd., Haicheng 114200, China |
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| Abstract: | In this study, six different macroporous adsorption resins were evaluated for their use to purify polyphenols from
Aronia melanocarpa berries. Macroporous resin XAD-7 was selected as the most suitable one. The purification parameters
optimized by static adsorption and desorption tests were as follows: adsorption and desorption time, 4 and 2 h, respectively;
sample concentration, 3.6 mg/mL; sample pH, 4; and desorption solvent, 95% ethanol (pH 7). The best purification
parameters optimized by dynamic adsorption and desorption tests were as follows: sample loading amount, 560 mL; flow
rate, 2 mL/min; washing the column with 350 mL of water; and subsequent elution with an eluent volume of 300 mL at
2 mL/min flow rate. Under these conditions, the purity of polyphenols from A. melanocarpa was improved from 11.62% to
64.37%, indicating that macroporous resin XAD-7 has a better effect in purifying polyphenols from A. melancarpa. |
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| Keywords: | Aronia melanocarpa polyphenols macroporous resin purification |
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