| PMP-HPLC-MS~n法分析弱酸降解鲍鱼性腺多糖产生的寡糖 |
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| 引用本文： | 鲁姣姣,艾春青,温成荣,徐 鑫,张 豹,宋 爽.PMP-HPLC-MS~n法分析弱酸降解鲍鱼性腺多糖产生的寡糖[J].食品科学,2016,37(16):112-116. |
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| 作者姓名： | 鲁姣姣 艾春青 温成荣 徐 鑫 张 豹 宋 爽 |
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| 作者单位： | 1.大连工业大学食品学院，辽宁 大连 116034；2.国家海洋食品工程技术研究中心，辽宁 大连 116034；;
3.海洋活性多糖开发应用技术国家地方联合工程实验室，辽宁 大连 116034 |
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| 基金项目： | 国家自然科学基金青年科学基金项目（31301431；31501439）；
辽宁省农业领域青年科技创新人才培养计划项目（2014002）；辽宁省教育厅一般项目（L2014222） |
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| 摘 要： | 为考察鲍鱼性腺硫酸多糖(abalone gonad sulfated polysaccharide,AGSP)在弱酸降解过程中产生的寡糖,对不同酸浓度的降解产物1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)衍生化后,进行高效液相色谱-质谱(high performance liquid chromatography-mass spectrometry,HPLC-MSn)法检测。在AGSP的弱酸降解产物中发现了2种二糖(disaccharides,DS1、DS2)和1种四糖(tetrasaccharide,TS)。2种二糖均是由己糖醛酸通过糖苷键连于己糖,其中DS2的结构确定为β-Glc A(1→2)-Man,而TS是由2个DS2连接而成。在100℃加热1 h的条件下,在0.1~2.0 mol/L的三氟乙酸浓度范围内,这3种寡糖的产率都随着酸浓度的增加呈升高趋势。
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| 关 键 词： | 鲍鱼 多糖 酸降解 质谱 寡糖 |
Analysis of Oligosaccharides Released from Weak Acid Hydrolysis of Abalone Gonad Polysaccharide Using PMP-HPLC-MSn |
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| LU Jiaojiao,AI Chunqing,WEN Chengrong,XU Xin,ZHANG Bao,SONG Shuang.Analysis of Oligosaccharides Released from Weak Acid Hydrolysis of Abalone Gonad Polysaccharide Using PMP-HPLC-MSn[J].Food Science,2016,37(16):112-116. |
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| Authors: | LU Jiaojiao AI Chunqing WEN Chengrong XU Xin ZHANG Bao SONG Shuang |
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| Affiliation: | 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. National Engineering Research;
Center of Seafood, Dalian 116034, China; 3. National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide;
Development and Application, Dalian 116034, China |
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| Abstract: | In order to investigate oligosaccharides released from abalone gonad sulfated polysaccharide (AGSP) through
weak acid hydrolysis, hydrolysates obtained at different acid concentrations were labeled with 1-phenyl-3-methyl-5-
pyrazolone (PMP), and then analyzed by high performance liquid chromatograph-mass spectrometry (HPLC-MSn). Two
disaccharides (DS1 and DS2) and one tetrasaccharide (TS) were found in the AGSP hydrolysates. The two disaccharides
were composed of a hexuronic acid linked to a hexose through a glucosidic bond. DS2 was deduced as β-GlcA(1→2)-Man,
and TS was proposed as a dimer of DS2. In hydrolysis reaction with 0.1–2.0 mol/L trifluoroacetic acid at 100 ℃ for 1 h, the
yields of the three oligosaccharides all increased with increasing acid concentration. |
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| Keywords: | abalone polysaccharide acid hydrolysis mass spectrometry oligosaccharide |
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