| 大孔树脂联合Sephadex LH-20葡聚糖凝胶分离纯化芦荟大黄素 |
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| 引用本文： | 张旭东,杨嘉宏,曹洁怡,麦玉平,王 亮.大孔树脂联合Sephadex LH-20葡聚糖凝胶分离纯化芦荟大黄素[J].食品安全质量检测技术,2019,10(23):7915-7920. |
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| 作者姓名： | 张旭东 杨嘉宏 曹洁怡 麦玉平 王 亮 |
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| 作者单位： | 吉林大学珠海学院, 药学与食品科学学院,吉林大学珠海学院, 药学与食品科学学院,吉林大学珠海学院, 药学与食品科学学院,吉林大学珠海学院, 药学与食品科学学院,吉林大学珠海学院, 药学与食品科学学院 |
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| 基金项目： | 广东省大学生创新创业培养计划(201713684021) |
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| 摘 要： | 目的 对芦荟药材中芦荟大黄素进行分离纯化及含量测定。方法 以DM301、X5、DM130 3种大孔树脂的静态吸附率, 动态吸附率为指标, 分离芦荟大黄素, 用Sephadex LH-20葡聚糖凝胶纯化芦荟大黄素, 采用高效液相色谱法(high performance liquid chromatography, HPLC)测定芦荟大黄素的含量。结果 芦荟大黄素线性回归方程为Y=41131X?44.34(r2=0.9993), DM301大孔树脂联合Sephadex LH-20葡聚糖凝胶分离纯化芦荟大黄素效果好。结论 该方法快速、简便, 为芦荟中有效成分大黄素分离和测定提供了依据,为大黄素的纯化提供了参考。
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| 关 键 词： | 芦荟大黄素 大孔树脂 Sephadex LH-20葡聚糖凝胶 分离纯化 高效液相色谱法 含量测定 |
| 收稿时间： | 2018/11/7 0:00:00 |
| 修稿时间： | 2019/11/22 0:00:00 |
Separation and purification of Aloe-emodin by macroporous resin and Sephadex LH-20 |
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| ZHANG Xu-Dong,YANG Jia-Hong,CAO Jie-Yi,MAI Yu-Ping and WANG Liang.Separation and purification of Aloe-emodin by macroporous resin and Sephadex LH-20[J].Food Safety and Quality Detection Technology,2019,10(23):7915-7920. |
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| Authors: | ZHANG Xu-Dong YANG Jia-Hong CAO Jie-Yi MAI Yu-Ping and WANG Liang |
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| Affiliation: | College of Pharmacy and Food Science, Zhuhai College of Jilin University,College of Pharmacy and Food Science, Zhuhai College of Jilin University,College of Pharmacy and Food Science, Zhuhai College of Jilin University,College of Pharmacy and Food Science, Zhuhai College of Jilin University and College of Pharmacy and Food Science, Zhuhai College of Jilin University |
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| Abstract: | Objective To isolate, purify and determine the content of aloe-emodin in aloe vera. Methods The static adsorption rate and dynamic adsorption rate of three macroporous resins of DM301, X5 and DM130 were used to separate aloe-emodin. Sephadex LH-20 glucan gel was used to purify aloe-emodin, and high performance liquid chromatography (HPLC) was used to determine aloe-emodin content. Results The linear regression equation of aloe-emodin was Y=41131X?44.3 (r2=0.9993). The separation and purification of aloe-emodin by DM301 macroporous resin combined with Sephadex LH-20 dextran gel was effective. Conclusion The method is rapid and simple, which provides a basis for the separation and determination of the active ingredient emodin in aloe, and a reference for purification. |
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| Keywords: | Aloe-emodin macroporous resin Sephadex LH-20 dextran gel separation and purification high performance liquid chromatography determination |
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