基于PHMG的丙烯酰胺/琼脂双网络抗菌水凝胶

以琼脂（Agar）、丙烯酰胺（Am）、单宁酸（TA）、氧化石墨烯（GO）为主要原料制备双网络水凝胶辅料。通过TA将GO还原成rGO，以TA/rGO为微反应器与聚六亚甲基胍酸盐（PHMG）反应，将其固定于水凝胶骨架中，制备双网络抗菌水凝胶。对凝胶进行力学性能、热力学性能、抗菌性能等测试。结果表明，琼脂的加入使凝胶的断裂伸长率提高了60%，抗压强度提高，凝胶内部孔隙进一步缩小，力学性能得到提高；质量分数为2.0%PHMG水溶液浸泡后得到的抗菌水凝胶对大肠杆菌和金黄色葡萄球菌的抑菌率均达到99.80%以上；无抑菌圈形成，说明PHMG被固定于凝胶中；红细胞（RBC）溶血实验表明凝胶溶血率均低于5%，抗菌水凝胶有着良好的生物相容性。

Preparation And Properties Of High Strength Antibacterial Hydrogel Based On Phmg

Dual-network high strength hydrogel excipients were prepared from agar, acrylamide, tannic acid (TA) and graphene oxide (GO). GO was reduced by TA and reacted with polyhexamethylene guanidinate (PHMG) in TA/rGO microreactor. It was immobilized in the hydrogel skeleton to prepare antimicrobial hydrogel excipients. The properties of the gels were tested by mechanical properties, thermodynamic properties and antibacterial properties. The results showed that the elongation at break of the gel increased by 60%, increase in compressive strength, the internal porosity of the gel was further reduced, and the mechanical properties were improved; When soaked in 2.0% PHMG aqueous solution, the antibacterial rate of E. coli and S. aureus reached above 99.80%; no bacteriostatic circle was formed, indicating that PHMG was immobilized in gel; Hemolysis of erythrocyte (RBC) showed that the gel hemolysis rate was lower than 5%, and the antibacterial hydrogel had good biocompatibility.