魔芋葡甘低聚糖对大鼠肠道环境的影响

目的：研究以半干法酶解制备的魔芋葡甘低聚糖（konjac oligosaccharides，KOS）对大鼠肠道环境的影响。方法：将KOS掺入到饲料中饲喂Sprague-Dawley（SD）大鼠，30 d后测定大鼠盲肠内容物各项指标和盲肠、小肠的理化指标。结果：KOS可以明显增加大鼠粪便含水率及盲肠壁表面积、小肠的拉伸性能、盲肠内容物中双歧杆菌和乳酸菌的数量；降低盲肠内容物pH值及含水率、游离氨及挥发性醛类和含氮类物质含量；抑制大肠杆菌和梭状芽孢杆菌的生长，增加短链脂肪酸的产生量。结论：KOS可以调节大鼠肠道菌群组成，改善肠道环境，促进肠道健康，是一种优良的益生元。

Effect of Konjac Oligosaccharides on Rat Intestinal Environment

Objective: To evaluate the effect of konjac oligosaccharides (KOS), prepared by semi-drying enzymatic
hydrolysis, on the rat intestinal environment. Methods: The cecal contents, cecal parameters and small intestinal parameters
of Sprague-Dawley rats were measured to evaluate the safety of KOS after 30 days of dietary incorporation with KOS.
Results: KOS could significantly increase the moisture content of rat feces and the surface area of the cecum wall, the tensile
strength of the small intestine, the total amounts of beneficial bacteria such as Bifidobacterium spp., Lactobacillus and reduce
pH and moisture, volatile aldehydes and nitrogenous substances contents of the cecal contents. In addition, KOS inhibited
the growth of Escherichia coli and Bacterium fusiformis and increased the total amount of short-chain fatty acids (SCFAs).
Conclusion: KOS can adjust the intestinal microflora, improve the intestinal environment and promote intestinal health as an
excellent prebiotic.