燕麦β-葡聚糖对2型糖尿病大鼠肠黏膜屏障的影响

目的:探究燕麦β-葡聚糖对2型糖尿病大鼠肠黏膜屏障的影响。方法:雄性SD大鼠高脂饲料喂养4周,腹腔注射链脲佐菌素(streptozotocin,STZ)建立2型糖尿病模型,成模后随机分为模型对照组和燕麦β-葡聚糖低、中、高剂量组。低、中、高剂量组由基础饲料添加燕麦β-葡聚糖至其质量分数分别为0.275%、0.550%、1.100%,模型对照组和正常对照组均以基础饲料喂养。10周后,测定其空腹血糖、肠道菌群以及结肠β-防御素-2、分泌型免疫球蛋白A(secretory immunoglobulin A,s Ig A)、紧密连接蛋白Occludin含量。结果:高剂量组较模型对照组血糖水平显著降低(P0.05);燕麦β-葡聚糖各干预组结肠紧密连接蛋白Occludin的表达水平显著高于模型对照组(P0.05),β-防御素-2水平显著低于模型对照组(P0.05),结肠s Ig A水平与模型对照组之间的差异不显著(P0.05);中、高剂量组结肠大肠杆菌数量上升幅度减小(P0.05),燕麦β-葡聚糖各干预组双歧杆菌、乳酸杆菌数量上升幅度增大(P0.05)。结论:燕麦β-葡聚糖可以改善2型糖尿病大鼠肠黏膜机械屏障和生物屏障的损伤,起到保护肠黏膜屏障的作用。

Effect of Oat β-Glucan on Intestinal Mucosal Barrier in Type 2 Diabetic Rats

Objective: To investigate the effect of oat β-glucan on intestinal mucosal barrier in type 2 diabetic rats. Methods:
Totally 60 male SD rats were randomly divided into five groups including normal control group and four diabetic model
groups. Type 2 diabetic rat models were established by high-fat diet feeding and low-dose intraperitoneal injection of
streptozotocin (STZ, 0.01 mol/L, 25 mg/(kg·d)). The diabetic rats were randomly divided into model control group and
low, medium and high dose oat β-glucan treatment group. Oat β-glucan was administrated by lavage. Low (0.275%),
medium (0.550%) and high (1.100%) doses of oat β-glucan added to the basal diet were given to each intervention group,
respectively. The rats in the model control and normal control groups were fed on the basal diet. After 10 weeks, fasting
plasma glucose, intestinal flora and colon β-defensin-2, secretory immunoglobulin A (sIgA) and occludin level were
measured. Results: Fasting plasma glucose of the high dose oat β-glucan treatment group was lower than that of the model
control group (P < 0.05). Compared with model control group, the expression level of the colonic tight junction protein
occludin was higher (P < 0.05), and β-defense-2 levels were lower (P < 0.05); yet no difference was found in sIgA levels
(P > 0.05) in oat β-glucan treatment groups. The increase in Escherichia coli counts in the medium and high dose oat β-glucan
treatment groups were smaller than that in the model control group (P < 0.05). The increase in bifidobacteria and lactobacilli
counts of oat β-glucan treatment groups were larger than that in the model control group (P < 0.05). Conclusion: Oat β-glucan
has a positive effect on intestinal mucosal mechanical barrier and biological barrier injury in type 2 diabetic rats.