2017~2018年云南省9种新鲜鱼类产品中4种硝基呋喃代谢物的检测

目的 对2017~2018年云南省9种鱼类水产品中的4种硝基呋喃类药物呋喃它定(furaltadon)、呋喃西林(furacilin)、呋喃妥因(titrofuranyoine)、呋喃唑酮(furazolidonum)的相关代谢物3-Amino-5-morpholinomethyl- 2-oxazolidinone(AMOZ)、1-Aminohydantoin(AHD)、Semicardazide(SEM)、3-Amino-2-oxazolidinone(AOZ)进行检测。方法 每年从云南昆明(9份)、曲靖(9份)、玉溪(9份)、昭通(9份)、红河(9份)、普洱(9份)、文山(9份)、德宏(9份)共8个地市的超市和农贸市场随机取样72份, 2年共采样144份。将样品搅碎后在酸性条件(盐酸水解)下用2-NBA衍生剂衍生一夜(约16 h), 采用液液萃取的方式净化, 利用超高效液相色谱-串联质谱仪测定。用内标法定量。结果 云南省部分水产品中硝基呋喃类药物残留水平超标。结论 此方法可对当地的食品安全提供保障。

Determination of 4 nitrofuran metabolites in 9 fresh fish products in Yunnan province from 2017 to 2018

Objective To test 3-amino-5-morpholinomethyll-2-oxazolidinone (AMOZ), 1-Aminohydantoin (AHD), semiconductor (SEM), 3-amino-2-oxazolidino (AOZ) related metabolites of 4 kinds of nitrofuran drugs furaltadon, furacillin, ttofuranoyoine, furazolidonum in 9 kinds of fish aquatic products of Yunnan province in 2017-2018. Methods 72 samples were randomly collected from supermarkets and farmers'' markets in 8 cities, including Kunming (9), Qujing (9), Yuxi (9), Zhaotong (9), Honghe (9), Pu''er (9), Wenshan (9), Dehong (9). 144 samples were collected in two years. The samples were stirred and then derivatized overnight (about 16 hours) with 2-nba derivatives under acid conditions (hydrochloric acid hydrolysis). The samples were purified by liquid-liquid extraction and determined by ultra performance liquid chromatography-tandem mass spectrometry. It was quantitative by internal standard method. Results The residue level of nitrofurans in some aquatic products of southern of Yunnan Province exceeded the standard. Conclusion This method can guarantee the local food safety.