广西牡蛎活性肽抑制卵巢癌淋巴结定向高转移细胞增殖及对运动迁移能力的调控作用

目的：研究广西牡蛎活性肽对卵巢癌淋巴结定向高转移细胞（SKOV3-PM4）的增殖及运动迁移能力的抑制作用。方法：采用四甲基偶氮唑蓝（3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide，MTT）法测定广西牡蛎活性肽作用24 h后对细胞增殖的抑制作用。细胞经不同质量浓度的广西牡蛎活性肽处理后，采用细胞计数法和集落形成实验测定细胞增殖能力；细胞划痕实验和Transwell小室测定细胞体外运动和侵袭迁移能力。结果：MTT实验结果显示牡蛎活性肽质量浓度在5～80 mg/L范围内抑制SKOV3-PM4的增殖，24 h的半数抑制浓度（inhibitory concentration 50%，IC50）为35.36 mg/L。细胞划痕实验表明无细胞毒性的质量浓度为4 mg/L的牡蛎活性肽能抑制SKOV3-PM4的增殖能力，牡蛎活性肽作用SKOV3-PM4细胞后其侵袭、迁移能力降低，与空白对照组比较差异显著（P＜0.05）。结论：从牡蛎中分离出的小分子多肽在一定质量浓度下对SKOV3-PM4细胞的生长增殖、运动迁移具有抑制作用。

Bioactive Peptide Derived from Oyster Meat from Guangxi Region,China Inhibits Proliferation and Migration of Human Ovarian Carcinoma Cells with Directional High Lymphatic Metastasis

Objective: To study the inhibitory effect of bioactive peptide derived from oyster meat Guangxi region,
China on the proliferation and migration of human ovarian carcinoma cells with directional high lymphatic metastasis
(SKOV3-PM4 cells). Methods: The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay
was performed to study the inhibitory effect of the bioactive peptide on cell proliferation in 24 h. The proliferative ability
of SKOV3-PM4 cells was detected by cell counting method and colony formation test. The ability of cell migration and
invasion was examined by cell wound scratch assay and matrigel invasion assay. Results: MTT results showed that the
bioactive peptide could inhibit the proliferation of SKOV3-PM4 within the concentration range of 5?80 mg/L and that half
inhibition concentration (IC50) in 24 hours was 35.36 mg/L. The cell growth curve and cell colony formation showed that
the proliferative ability of SKOV3-PM4 cells was significantly inhibited (P < 0.05). Compared with control cells, after
being treated with 4 mg/L (non-cytotoxic concentration) the bioactive peptide, the migration and invasion capability of
SKOV3-PM4 was also significantly decreased (P < 0.05). Conclusion: The bioactive peptide derived from oyster from
Guangxi region can inhibit the proliferation and migration of SKOV3-PM4 cells within a certain concentration range.