大孔吸附树脂-聚酰胺联用纯化茅岩莓总黄酮工艺优化

为纯化茅岩莓总黄酮，先用HPD-100型大孔吸附树脂（macroporous adsorption resin，MAR）层析柱进行初步纯化，再用聚酰胺（polyamide，PA）层析柱进行第2次纯化，得到的HPD-100型MAR最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速1 mL/min、上样液体积130 mL，在此条件下吸附率为97.14%；最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积40 mL，在此条件下解吸率为94.10%。经HPD-100型MAR纯化后的总黄酮纯度从55.00%提高到了72.25%。PA的最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速2 mL/min，在此条件下吸附率为99.57%；最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积55 mL，在此条件下解吸率为76.50%。经PA纯化后总黄酮纯度从72.25%提高到了80.75%。该方法为茅岩莓黄酮的纯化提供了一种更高效的方法，具有良好的应用前景。

Purification of Total Flavonoids from Ampelopsis grossedentata by Combined Use of Macroporous Adsorption Resin and Polyamide

Sequential column chromatographies on HPD-100 macroporous adsorption resin (MAR) and polyamide (PA)
were used to purify total flavonoids from Ampelopsis grossedentata. The optimum adsorption parameters for HPD-100 MAR
were determined as follows: sample concentration, 6 mg/mL; sample flow rate, 1 mL/min; and sample volume, 130 mL.
Under these conditions, the percentage adsorption was 97.14%. The optimum desorption parameters were established as
follows: eluent concentration, aqueous ethanol (70:30, V/V); eluent volume, 40 mL; and flow rate, 1 mL/min. Under these
conditions, the percentage desorption was 94.10%. After the treatment with HPD-100 MAR, the purity of total flavonoids
was increased from 55.00% to 72.25%. The optimum adsorption parameters for PA were determined as follows: sample
concentration, 6 mg/mL; and flow rate, 2 mL/min. Under these conditions, the percentage adsorption was 99.57%. The
optimum desorption parameters were determined as follows: 70% ethanol in water (V/V) as the eluent; eluent volume,
55 mL; and flow rate, 1 mL/min. Under these conditions, the percentage desorption was 76.50%. After the treatment with
PA, the purity of total flavonoids was further increased to 80.75%. The method presented in this study can provided a
promising approach for the efficiency purification of total flavonoids from Ampelopsis grossedentata.