红枣膳食纤维改善小鼠功能性便秘及调节肠道菌群功能

研究红枣膳食纤维改善小鼠功能性便秘和调节肠道菌群功能的作用。将小鼠随机分为溶剂对照组、模型对照组和0.30、0.60、1.20 g/(kg·d)低、中、高3个红枣膳食纤维剂量组。连续灌胃10 d后建立功能性便秘模型,记录红枣膳食纤维对小鼠小肠推进率、排便时间和排便质量的影响。按照同样的分组方法将小鼠分为4个组(溶剂对照组及红枣膳食纤维低、中、高剂量组),连续灌胃14 d,分别采集小鼠灌胃0 d和14 d的新鲜粪便进行培养和菌群鉴定、计数。结果显示:红枣膳食纤维可以极显著提高小鼠小肠推进率(P0.01),可缩短小鼠首粒排黑便时间并增加排便粒数和质量(P0.05);显著降低小鼠肠道内产气荚膜梭菌、肠杆菌和肠球菌的含量,同时促进双歧杆菌和乳杆菌的增殖(P0.05),并且在剂量达到0.60 g/(kg·d)时即能产生作用。可见红枣膳食纤维可改善小鼠功能性便秘、调节肠道菌群平衡。

Dietary Fiber of Red Jujube Ameliorates Functional Constipation and Improves Intestinal Health in Mice

The goal of this research was to explore the ameliorative effect of dietary fiber of red jujube (DFRJ) on functional constipation and its improvement effect on intestinal health in mice. Mice were divided into 5 groups as follows: blank control, model control, and low dose, middle dose and high dose DFRJ groups (0.30, 0.60 and 1.20 g/(kg·d)). A mouse model of functional constipation was established after oral administration of compound diphenoxylate for ten days. The intestinal motion rate, defecation time and feces weight of all the mice were investigated. Another 48 mice were chosen and allocated to the blank control group and the three dose groups, respectively, and orally administered for fourteen days. The fresh feces of the mice were collected before and after administration for 1 and 14 days, homogenized and diluted, and then the bacteria were identified and counted after cultivation. The results showed that DFRJ could significantly improve the intestinal propulsion rate of the mice (P < 0.01), shorten the excretion time of the first black feces and increase the number of defecation and the weight of feces (P < 0.05). DFRJ could significantly reduce the numbers of intestinal Escherichia coli, Enterococcus, and Clostridium perfringens, while it could promote the reproduction of Bifidobacterium and Lactobacillus (P < 0.05) at the dose of 0.60 g/(kg·d). Red jujube dietary fiber has the capacity to promote defecation and improve the intestinal flora in mice, which is beneficial for relieving functional constipation and enhancing intestinal health.