黄酒中9 种生物胺的高效液相色谱分析法

建立一种检测黄酒中生物胺的反相高效液相色谱法。用三氯乙酸提取黄酒中生物胺，丹磺酰氯作为衍生化试剂，1,7-二氨基庚烷作为内标定量。色谱条件为：Waters XBridge C18色谱柱（4.5 mm×250 mm，3.8 μm）作为分离柱，乙腈和水作为流动相进行梯度洗脱，流速1 mL/min，紫外检测波长254 nm。结果表明，9 种生物胺（盐酸吡哆胺、色胺、腐胺、尸胺、β-苯乙胺、组胺、酪胺、精胺和亚精胺）在40 min内能被很好分离。在1～50 mg/L质量浓度范围内，各种生物胺呈现良好的线性相关性（R2＞0.999）。各生物胺加标回收率为79.54%～89.55%，相对标准偏差为3.14%～6.35%，该法各组分检出限（RSN=3）为0.002～0.009 mg/L。应用柱前衍生化高效液相色谱-紫外检测法，实现了黄酒中生物胺的准确检测。

Development of an Analytical Method for Nine Biogenic Amines in Chinese Yellow Wine by HPLC

An analytical method for biogenic amines in Chinese rice wine by reversed phase high-performance liquid
chromatography (RP-HPLC-HPLC) was developed. The biogenic amines in Chinese yellow wine were extracted with
trichloroacetic acid (TCA), and then pre-column derivatized with dansyl chloride for quantification using 1,7-diamino
heptane as the quantitative internal standard. A C18 chromatographic column (4.5 mm × 250 mm, 3.8 μm) was used
for separation. The mobile phase was composed of a mixture of acetonitrile and water at a flow rate of 1 mL/min, and
the ultraviolet (UV) detection wavelength was 254 nm. The results showed that nine biogenic amines (pyridoxamine
dihydrochloride, tryptamine, putrescine, cadaverine, beta phenethylamine, histamine, tyramine, spermine and spermine)
were separated completely within 40 minutes. In the concentration range of 1–50 mg/L, each biogenic amine presented good
linear correlation (R2 > 0.999). After the addition of a mixed standard solution of biogenic amines to Chinese yellow wine,
the recovery rates were 79.54%–89.55%, with relative standard deviations (RSDs) ranging from 3.14% to 6.35%, and the
limit of detection for each component was 0.002–0.009 mg/L. Therefore, The HPLC method with pre-column derivatization
and UV detection could be used for the determination of biogenic amines in Chinese yellow wine.