Investigation of carbon nanofibers as support for bioactive substances |
| |
| Authors: | Liliana Olenic Gheorghe Mihailescu Stela Pruneanu Dan Lupu Alexandru R Biris Petre Margineanu Sorina Garabagiu Alexandru S Biris |
| |
| Affiliation: | (1) National Institute for R&D of Isotopic and Molecular Technologies, Donath Str, No.71-103, P.O. Box 700, Cluj-Napoca, Romania;(2) School of Natural Sciences, Newcastle University, Newcastle, NE1 7RU, UK;(3) Nanotechnology Center and Applied Science Department, University of Arkansas at Little Rock, Little Rock, AR 72204, USA |
| |
| Abstract: | In this paper we have studied the adsorption properties of various bio-active systems onto the surface of carbon nanofibers
(CNF) synthesized by chemical vapor deposition (CVD). Amino acids (alanine, aspartic acid, glutamic acid) and glucose oxidase
(GOx) were adsorbed on CNF and the results were compared with those obtained when activated carbon (AC) was used as support.
CNF and AC properties (hydrophilic or hydrophobic properties) were characterized by the pH value, the concentration of acidic/basic
sites and by naphthalene adsorption. CNF with immobilized GOx was additionally investigated as a highly sensitive glucose
biosensor. An amperometric method was used in an original manner to detect the changes in the specific activity of GOx, immobilized
longer time on CNF. The method demonstrates that not the whole enzyme adsorbed onto CNF can catalyze the oxidation of glucose
from the solution. |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
