| 稳定表达HIV-1病毒样颗粒的293细胞系的筛选及其稳定性 |
| |
| 引用本文： | 张喜珍,于湘晖,赵东海,谷铁军,姜春来,吴永革,孔维.稳定表达HIV-1病毒样颗粒的293细胞系的筛选及其稳定性[J].粉末涂料与涂装,2007,20(3):187-189. |
| |
| 作者姓名： | 张喜珍 于湘晖 赵东海 谷铁军 姜春来 吴永革 孔维 |
| |
| 作者单位： | 吉林大学生命科学学院疫苗研究中心 长春130012 |
| |
| 摘 要： | 目的获得高效、稳定表达含有HIV-1主要结构蛋白gag、pol和env的病毒样颗粒的293细胞系。方法选择合适的真核细胞表达质粒与带有抗性基因的载体,共转染293真核细胞,用含有抗性的培养基筛选出稳定表达细胞株。结果HIV-1的gag、pol和env蛋白均在293细胞中获得了表达,且不同代次的293细胞中蛋白表达情况无明显差异。结论成功筛选了稳定表达HIV-1病毒样颗粒的293细胞系,为进一步开发艾滋病疫苗奠定了基础。
|
| 关 键 词： | 人免疫缺陷病毒 病毒样颗粒 细胞系 |
| 文章编号： | 1004-5503（2007）03-187-03 |
| 收稿时间： | 2006-04-04 |
| 修稿时间： | 2006年4月4日 |
Screening and Stability of 293 Cell Line for Stable Expression of Virus-like Particles of HIV-1 |
| |
| ZHANG Xi-zhen, YU Xiang-hui, ZHAO Dong-hai, et al.Screening and Stability of 293 Cell Line for Stable Expression of Virus-like Particles of HIV-1[J].Chinese Journal of Biologicals,2007,20(3):187-189. |
| |
| Authors: | ZHANG Xi-zhen YU Xiang-hui ZHAO Dong-hai |
| |
| Affiliation: | Vaccine Research Center,College of Life Science,Jilin University, Changchun 130012, China |
| |
| Abstract: | Objective To screen the 293 cell line for stable and high expression of virus-like particles(VLPs) containing structural proteins gag,pol and env of HIV-1.Methods Co-transfect 293 cell line with eukaryotic expression vector D-GPEi for HIV-1 gag,pol and env genes and plasmid pcDNA3.1 carrying neomycin resistant gene,and screen the cell lines for stable expression with G418 resistant medium.Results The major structural proteins gag,pol and env of HIV-1 were expressed in 293 cells.The expression levels in screened cell lines of various passages showed no significant difference.Conclusion The 293 cell lines for stable expression of VLPs of HIV-1 were successfully screened,which laid a foundation of development of HIV vaccine. |
| |
| Keywords: | Human immunodeficiency virus(HIV) Virus-like particle(VLP) Cell line |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
