Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography |
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| Authors: | Zeynep Koksal Ramazan Kalin Ilhami Gulcin Hasan Ozdemir |
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| Affiliation: | 1. Faculty of Engineering and Natural Sciences, Department of Chemistry, Istanbul Medeniyet University, Istanbul, Turkey;2. Faculty of Science, Department of Basic Science, Erzurum Technical University, Erzurum, Turkey;3. Faculty of Science, Department of Chemistry, Ataturk University, Erzurum, Turkey;4. Faculty of Science, Department of Chemistry, Ataturk University, Erzurum, Turkey |
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| Abstract: | The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2,4-d-acid dimethylamine, fenoxaprop-p-ethyl, glyphosate isopropylamine, haloxyfop-p-methyl, cypermethrin, λ-cyhalothrin, and dichlorvos on the peroxidase purified from turnip (Brassica rapa L.) and black radish (Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, λ-cyhalothrin showed the strongest inhibitory effect against turnip peroxidase (Ki: 1.23 × 10?2 ± 0.21 × 10?2 mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase (Ki: 2.14 × 10?2 ± 0.08 × 10?2 mM) as competitive inhibition. |
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| Keywords: | Plant peroxidase Radish (Raphanus sativus) Turnip (Brassica rapa) Pesticides Enzyme inhibition |
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