Isolation and identification of new strains of crude oil degrading bacteria from Kharg Island,Iran |
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Authors: | Kazem Godini Mohamad Reza Samarghandi Doustmorad Zafari Ali Reza Rahmani Abbas Afkhami |
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Affiliation: | 1. Department of Environmental Health Engineering, Faculty of Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran;2. Department of Plant Protection, Faculty of Agriculture, University of Bu-Ali Sina, Hamadan, Iran;3. Department of Analytical Chemistry, Bu-Ali Sina University, Hamadan, Iran |
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Abstract: | Petroleum-degrading bacteria were isolated from polluted sites in Kharg Island, which is one of the most important ports for oil export in Iran. Both biochemical tests and 16S rRNA were applied to identify the strains. Experiments were performed in a mineral salt medium containing 2% of crude oil as the sole carbon. The results illustrated that the strains were: Brevibacillus sp., Microbacterium oxydans, Staphylococcus arlettae, Staphylococcus warneri, Methylobacterium persicinum, and Achromobacter xylosoxidans. Growth rates of these strains by its optical densities at the wavelength of 600 illustrated that Pseudomonas sp. (accession no. KC771232) had the highest growth rate (OD600 0.8). |
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Keywords: | bacterial strains growth rate petroleum compounds pseudomonas sp 16S rRNA |
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