| 食物过敏原蛋白的定量检测方法 |
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| 引用本文： | 闫 飞,廖志勇,吴志华,陈红兵.食物过敏原蛋白的定量检测方法[J].食品科学,2010,31(19):426-429. |
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| 作者姓名： | 闫 飞 廖志勇 吴志华 陈红兵 |
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| 作者单位： | 1.南昌大学 食品科学与技术国家重点实验室，中德联合研究院 2.温州大学生命与环境科学学院 |
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| 基金项目： | 江西省自然科学基金项目(2007GQN2001)；江西省教育厅科技计划项目(赣教技字[GJJ08052]号) |
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| 摘 要： | 食物过敏现象的日益严重凸显食物过敏原蛋白定量检测的重要性。目前对食物过敏原蛋白进行定量检测的方法多是基于单个蛋白的间接测定。随着质谱分析方法的进步，定量蛋白质组学技术已开始并将广泛应用于复杂食品混合物中微量过敏原的直接定量检测。本文分别对基于单个蛋白和蛋白质组学的食物过敏原蛋白的定量测定方法进行综述。
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| 关 键 词： | 定量 过敏原 质谱 蛋白质组学 |
| 收稿时间： | 2010-06-30 |
A Review of Quantitative Detection of Food Allergens |
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| YAN Fei,LIAO Zhi-yong,WU Zhi-hua,CHEN Hong-bing.A Review of Quantitative Detection of Food Allergens[J].Food Science,2010,31(19):426-429. |
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| Authors: | YAN Fei LIAO Zhi-yong WU Zhi-hua CHEN Hong-bing |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University,
Nanchang 330047, China；2. College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China |
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| Abstract: | The increasing phenomena of food allergy have highlighted the importance of quantitative analysis of food allergens. Currently, most methods for the quantitative detection of food allergens are indirect methods based on a single protein. Due to the development of mass spectrometry, quantitative proteomics is being widely used for the direct quantitative detection of trace allergens in complex food mixtures. In this paper, the research progress in quantitative detection methods for food allergens is discussed. |
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| Keywords: | quantification food allergen mass spectrometry (MS) proteomics |
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