固相萃取-流动注射化学发光分析法测定鸡蛋中的沙拉沙星

建立一种简便、高灵敏度的测定鸡蛋中沙拉沙星的新方法。基于沙拉沙星对鲁米诺- 铁氰化钾化学发光体系有显著增敏作用的研究，鸡蛋样品中的沙拉沙星用磷酸盐提取，固相萃取C18 小柱净化，结合流动注射技术和化学发光检测，建立测定鸡蛋中沙拉沙星的新方法。结果表明，方法的线性范围为6 × 10-9～6 × 10-5mol/L，检出限为3.97 × 10-11mol/L，平均回收率为96.2%，RSD 为1.71%(n=11)，结果令人满意。

Use of Solid Phase Extraction - Flow Injection- Chemiluminescence for Determination of Sarafloxacin in Eggs

A high sensitive and fast method was developed for determination of sarafloxacin(SAR) in eggs. Sarafloxacin in sample was extracted with phosphate, and purified by solid phase extraction (SPE). Since sarafloxacin could significantly enhanced the reaction between luminol and potassium ferricyanide, it could be detected by flow injection-chemiluminescence method. The linear ranges of this method was 6×10-9－6×10-5 mol/L, and the detection limit was 3.97×10-11 mol/L. The average recovery was 96.2%, and the relative stand deviation (RSD) was 1.71%(n=11).