双歧杆菌RH 菌株不同处理物修复肠道菌群平衡失调的研究

为探讨双歧杆菌RH 菌株对肠道菌群平衡失调的修复功能，本研究通过动物实验，考察双歧杆菌活菌(LB)、菌体破碎物(CE)及发酵上清液(FS)3 种处理物灌胃由抗生素造成的菌群失调模型小鼠后对其肠道内双歧杆菌、乳杆菌、产气荚膜梭菌、肠球菌、肠杆菌和拟杆菌6 大类主要菌群的影响。结果显示：3 种处理物均可使小鼠肠道内双歧杆菌和乳杆菌数量极显著增加(P ＜ 0.01)，产气荚膜梭菌和肠球菌数量显著降低(P ＜ 0.05 和P ＜ 0.01)，并能使肠杆菌和拟杆菌数量恢复至正常水平，说明该双歧杆菌LB、CE 和FS 均可起到修复、调节小鼠肠道微生态平衡的作用。

Restoration Capability of Bifidobacteria with Different Treatments on Imbalanced Flora in Intestine

In order to explore the restoration capability of Bifidobacteria on imbalanced flora in intestine, live bacteria (LB), cell-free extract (CE) and fermentation supernatant (FS) of Bifidobacteria were used to treat the vancomycin-induced mice model with imbalanced flora by gastric gavage. The effects of three different treatments of Bifidobacteria on major species of intestinal flora were evaluated. Results showed that three different treatments could significantly increase the proliferation of Bifidobacteria and Lactobacillia (P ＜ 0.01) and decrease the amounts of Clostridium perfringens and Enterococcus (P ＜ 0.05 and P ＜0.01). Bacteroide and Enterobacteriaceae were increased to the normal level. Therefore, LB, CE and FS of Bifidobacterium RH could restore and modulate the intestinal microecological balance of mice.