气相色谱- 质谱法分离鉴别溪黄草叶中的弱极性有机组分

利用气相色谱- 质谱法分析石油醚相及乙酸乙酯相中弱极性有机组分是否含有与抗氧化性有关的化合物。通过制备溪黄草叶60% 乙醇提取物，并采用溶剂分离法，从溪黄草叶提取物中分出4 个组分：石油醚相、乙酸乙酯相、正丁醇相、水相。气相色谱- 质谱法共鉴定出15 种成分，其中大多数为高级脂肪酸，尤其以棕榈酸、亚麻酸甲酯含量最为丰富。虽从溪黄草叶石油醚相中鉴定出2,2'- 亚甲基双-(4- 甲基-6- 叔丁基苯酚)(工业强抗氧化剂)，但其含量很低。

Gas Chromatography - Mass Spectrometry Separation and Identification of Organic Weak Polar Components from Rabdosia serra (Maxim.) Hara Leaves

Rabdosia serra (Maxim.) Hara leaves were extracted with 60% ethanol, and were further separated by solvent extraction into four fractions, i.e., petroleum ether fraction, ethyl acetate fraction, n-butanol fraction and water fraction. Components in petroleum ether and ethyl acetate fractions were analyzed by using gas chromatography-mass spectrometry. Totally 15 compounds were identified, and most of these compounds are higher fatty acids, especially n-hexadecanoic acid and 9,12,15-octadecatrienoic acid. Trace 2,2'-methylene bis(6-tert-butyl-4-methylphenol), a strong antioxidant, was found in the petroleum ether fraction.