Chemical composition and antioxidant activity of tronchuda cabbage internal leaves |
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Authors: | Federico Ferreres Carla Sousa Vendula Vrchovská Patrícia Valentão José A Pereira Rosa M Seabra Paula B Andrade |
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Affiliation: | 1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Campus University Espinardo, P.O. Box 164, 30100, Murcia, Spain 2. REQUIMTE/ Servi?o de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047, Porto, Portugal 3. Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic 4. CIMO/ESAB, Quinta de Sta Apolónia, Apartado 1172, 5301-855, Bragan?a, Portugal
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Abstract: | A phytochemical study was undertaken on the internal leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC). Seventeen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-ESI-MS
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and HPLC/DAD, respectively: quercetin 3-O-sophoroside-7-O-glucoside, 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)-sophoroside-7-O-glucoside, sinapoyl gluc-oside acid, kaempferol 3-O-(sinapoyl)-sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl)-sophoroside-7-O-glucoside, kaempferol 3-O-(p-coumaroyl)-sophoroside-7-O-glucoside, 4-p-coumaroylquinic acid, sinapic acid, kaempferol 3-O-sophoroside, 3 isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2-trisinapoylgentiobiose
and 1,2′-disinapoyl-2-ferul-oylgentiobiose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric
acids) were also identified and quantified. The hot water extract of tronchuda cabbage internal leaves was investigated for
its capacity to act as a scavenger of DPPH• radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant
capacity in a concentration dependent manner against all radicals. |
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Keywords: | Tronchuda cabbage internal leaves Brassica oleracea L var costata DC Phenolics Organic acids Antioxidant capacity |
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