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| 高效液相色谱法测定牛奶中的胆固醇 | |
| 引用本文： | 张丽,汤佳佳,徐国兵,耿超. 高效液相色谱法测定牛奶中的胆固醇[J]. 食品科学, 2012, 33(2): 216-218. DOI: 10.7506/spkx1002-6630-201702047 |
| 作者姓名： | 张丽 汤佳佳 徐国兵 耿超 |
| 作者单位： | 1.安徽中医学院药学院 2.安徽省食品药品检验所 |
| 摘 要： | 目的：建立高效液相色谱(high performance liquid chromatography，HPLC)法测定牛奶中胆固醇的方法。方法：色谱柱为Shimadzu C18柱(4.6mm×250mm，5μm)，流动相为乙腈，流速为1.0mL/min，检测波长为203nm，柱温为30℃。结果：胆固醇的线性范围为3.58～358μg/mL(r＝0.9999)，平均回收率为101.8%(n＝6)，相对标准偏差为1.14%。结论：该方法测定结果准确、重现性好，可作为测定牛奶中胆固醇含量的方法。 |
| 关 键 词： | 高效液相色谱法 牛奶 胆固醇 |
| 收稿时间： | 2017-02-16 |
Determination of Cholesterol in Milk by HPLC |
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| ZHANG Li,TANG Jia-jia,XU Guo-bing,GENG Chao. Determination of Cholesterol in Milk by HPLC[J]. Food Science, 2012, 33(2): 216-218. DOI: 10.7506/spkx1002-6630-201702047 | |
| Authors: | ZHANG Li TANG Jia-jia XU Guo-bing GENG Chao |
| Affiliation: | 1. College of Pharmacy, Anhui University of Chinese Traditional Medicine, Hefei 230031, China；2. Anhui Institute for Food and Drug Control, Hefei 230051, China |
| Abstract: | Objective: To establish an HPLC method for the determination of cholesterol in milk.Methods: Cholesterol was separated on a Shimadzu C18column(4.6 mm × 250 mm,5μm) using acetonitrile as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm and the column temperature at 30 ℃.Results: A calibration curve was established revealing a good linear relationship in the range of 3.58-358μg/mL with a correlation coefficient of 0.9999.The average spike recovery rate was 101.8% with a RSD of 1.14%.Conclusion: The method was accurate,reproducible and applicable for the determination of cholesterol in milk. |
| Keywords: | high performance liquid chromatography(HPLC) milk cholesterol |
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