Immobilization cyclen copper (II) on merrifield resin: Efficient oxidative cleavage of plasmid DNA

Merrifield resin‐supported cyclen (MRC) was directly prepared by attaching 1, 4, 7, 10‐tetraazacyclododecane (cyclen) to Merrifield resin (MR). Subsequent coordination with Cu(II), Co(II), and Ni(II) gave immobilized cyclen complex MRC‐Cu, MRC‐Co, and MRC‐Ni as “solid artificial enzymes.” These complexes were characterized by elemental analysis, IR spectroscopy, ICP‐AES, and scanning electron microscopy (SEM). Furthermore, the DNA cleavage activities of these complexes were investigated by agarose gel electrophoresis. The results indicated that MRC‐Cu was superior to other solid artificial enzymes, and the cleavage process was carried out via oxidative pathway. Moreover, the solid catalyst MRC‐Cu was very stable and it could be reused at least four times without any loss of catalytic activity. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009