Enzyme‐Mediated Deposition of a TiO2 Coating onto Biofunctionalized WS2 Chalcogenide Nanotubes |
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| Authors: | Muhammad Nawaz Tahir Filipe Natalio Helen Annal Therese Aswani Yella Nadine Metz Muhammad Raza Shah Enrico Mugnaioli Rüdiger Berger Patrick Theato Heinz‐Christoph Schröder Werner E. G. Müller Wolfgang Tremel |
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| Affiliation: | 1. Institut für Anorganische Chemie und Analytische Chemie Johannes‐Gutenberg‐Universit?t Duesbergweg 10–14, 55099 Mainz (Germany);2. Institut für Physiologische Chemie Abteilung Angewandte Molekularbiologie Universit?t Mainz Duesbergweg 6, 55099 Mainz (Germany);3. Institut für Organische Chemie Johannes‐Gutenberg‐Universit?t Duesbergweg 10–14, 55099 Mainz (Germany);4. Max‐Planck‐Institut für Polymerforschung Ackermannweg 10, 55128 Mainz (Germany);5. Institut für Physikalische Chemie Johannes‐Gutenberg‐Universit?t Welderweg 11, 55099 Mainz (Germany) |
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| Abstract: | A chemically specific and facile method for the biofunctionalization of WS2 nanotubes (NT‐WS2) is reported. The covalent modification strategy is based on the affinity of the nitrilotriacetic acid (NTA) side chain, which serves as a ligand for the surface binding to NT‐WS2 and simultaneously as an anchor group for the binding of His‐tagged proteins to the polymer backbone. The polymer functionalized WS2 nanotubes can be solubilized either in water or organic solvents; they are stable for at least one week. The probes were characterized by FT‐IR and UV‐vis spectroscopy. The immobilization of silicatein, a hydrolytic protein encountered in marine sponges, was visualized by scanning force microscopy (SFM) and confocal laser scanning microscopy (CLSM). The formation of the biotitania coating mediated by the immobilized silicatein onto the surface was characterized by scanning electron microscopy (SEM), and transmission electron microscopy (TEM). |
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| Keywords: | biofunctionalization layered chalcogenides nanotubes silicatein WS2 |
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