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Insights into Sequence–Activity Relationships amongst Baeyer–Villiger Monooxygenases as Revealed by the Intragenomic Complement of Enzymes from Rhodococcus jostii RHA1 |
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| Authors: | Claudia Szolkowy Lindsay D. Eltis Prof. Dr. Neil C. Bruce Prof. Dr. Gideon Grogan Dr. |
| Affiliation: | 1. York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5YW (UK), Fax: (+44)?1904‐328266;2. Department of Microbiology and Immunology, Life Sciences Centre, University of British Columbia, 2350 Health Sciences Mall, Vancouver V6T 1Z3, BC (Canada);3. Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5YW (UK) |
| Abstract: | The Rhodococcus jostii RHA1 genome encodes a number of enzymes that can be exploited as biocatalysts. Study of the substrate spectrum and enantioselectivity of Baeyer–Villiger monooxygenases from R. jostii allowed the identification of short amino acid sequences specific to groups displaying certain catalytic characteristics. The gel illustrates the substrate acceptance spectra and selectivities of the different proteins.  |
| Keywords: | Baeyer– Villiger reaction enzyme catalysis ketones oxidoreductases stereoselectivity |