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Analysis of Protein–Protein Interactions by Using Droplet‐Based Microfluidics |
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| Authors: | Monpichar Srisa‐Art Dong‐Ku Kang Dr. Jongin Hong Dr. Hyun Park Robin J. Leatherbarrow Prof. Joshua B. Edel Dr. Soo‐Ik Chang Prof. Andrew J. deMello Prof. |
| Affiliation: | 1. Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ (UK), Fax: (+44)?207‐594‐5801;2. Department of Biochemistry, Protein Chip Research Centre Chungbuk National University, Cheongju, Chungbuk 361–763 (Korea), Fax: (+82)?43‐267‐2306;3. Institute of Biomedical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ (UK), Fax: (+44)?207‐594‐5196 |
| Abstract: | Every little drop : The KD values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL‐sized droplets agree with data from bulk‐fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti‐ANG antibodies to ANG. Such an experimental platform could be applied to the high‐throughput analysis of protein–protein interactions.  |
| Keywords: | angiogenin antibody angiogenin microdroplets microfluidics protein– protein interactions |