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Observing Proteins as Single Molecules Encapsulated in Surface‐Tethered Polymeric Nanocontainers |
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| Authors: | Tobias Rosenkranz Alexandros Katranidis Diaa Atta Ingo Gregor? Dr Jörg Enderlein Prof Mariusz Grzelakowski Dr Per Rigler Dr Wolfgang Meier Prof Jörg Fitter Dr |
| Affiliation: | 1. Forschungszentrum Jülich, INB‐2, Molecular Biophysics, 52425 Jülich (Germany), Fax: (+49)?2461‐612020;2. Laboratory of Biochemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki (Greece);3. Forschungszentrum Jülich, INB‐1, Cellular Biophysics, 52425 Jülich (Germany);4. Present address: Forschungszentrum Caesar, Abteilung Molekulare Neurosensorik, Ludwig‐Erhard‐Allee 2, 53175 Bonn (Germany);5. Institut für physikalische und theoretische Chemie, Eberhard Karls Universit?t Tübingen, 72076 Tübingen (Germany);6. Institut für Physikalische Chemie, Universit?t Basel, Klingelbergstrasse 80, 4056 Basel (Switzerland) |
| Abstract: | Protein unfolding inside immobilized polymerosomes : One of the most interesting properties of polymeric vesicles is their remarkable stability against extreme temperatures and osmotic stress, and their longevity even under harsh environmental conditions. We have demonstrated, in an application on protein folding, that surface‐tethered polymerosomes are suitable for performing time‐resolved single molecule studies with encapsulated proteins, as illustrated here.  |
| Keywords: | fluorescence spectroscopy photoinduced electron transfer protein encapsulation protein folding single‐molecule studies |