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Altering the Substrate Specificity of RhlI by Directed Evolution |
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| Authors: | Pavan Kumar Reddy Kambam Dawn T. Eriksen Jason Lajoie Daniel J. Sayut Lianhong Sun Prof. Dr. |
| Affiliation: | Department of Chemical Engineering Department, University of Massachusetts, 686 N. Pleasant Street, Amherst, MA 01003 (USA), Fax: (+1)?413‐5456143 |
| Abstract: | Reducing virulence : RhlI catalyzes the synthesis of N‐butanoyl homoserine lactone (BHL), with a minor product N‐hexanoyl homoserine lactone (HHL). By using directed evolution and a genetic screen, RhlI has been engineered for enhanced production of both BHL and HHL at a similar level.  |
| Keywords: | directed evolution genetic engineering high‐throughput screening metabolism quorum sensing |