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Altering the Substrate Specificity of RhlI by Directed Evolution
Authors:Pavan Kumar Reddy Kambam  Dawn T. Eriksen  Jason Lajoie  Daniel J. Sayut  Lianhong Sun Prof. Dr.
Affiliation:Department of Chemical Engineering Department, University of Massachusetts, 686 N. Pleasant Street, Amherst, MA 01003 (USA), Fax: (+1)?413‐5456143
Abstract:Reducing virulence : RhlI catalyzes the synthesis of N‐butanoyl homoserine lactone (BHL), with a minor product N‐hexanoyl homoserine lactone (HHL). By using directed evolution and a genetic screen, RhlI has been engineered for enhanced production of both BHL and HHL at a similar level.
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Keywords:directed evolution  genetic engineering  high‐throughput screening  metabolism  quorum sensing
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