Synthesis of a photoimmobilizable histidine polymer for surface modification |
| |
| Authors: | Makoto Sakuragi Saki Tsuzuki Hirokazu Hasuda Akira Wada Kenji Matoba Izumi Kubo Yoshihiro Ito |
| |
| Affiliation: | 1. Nano Medical Engineering Laboratory, Riken, 2‐1 Hirosawa, Wako‐Shi, Saitama 351‐0198, Japan;2. Regenerative Medical Bioreactor Project, Kanagawa Academy of Science and Technology, KSP East 309, 3‐2‐1 Sakado, Takatsu‐ku, Kawasaki‐shi, Kanagawa 213‐0012, Japan;3. Department of Bioinformatics, Graduate School of Engineering, Soka University, 1‐236 Tangi‐cho, Hachioji‐shi, Tokyo 192‐8577, Japan |
| |
| Abstract: | A novel photoreactive polymer with histidine polar groups was synthesized through the copolymerization of two types of methacrylic acid, one carrying histidine groups and the other carrying azidoaniline groups. The polymer was photoimmobilized on polyester disks for surface modification. The effect of the surface modification on the hydrophilic and biofouling properties was investigated. Static contact angle measurements showed that the polymeric surface was modified to be comparatively hydrophilic in the polymer‐immobilized region. Micropattern immobilization was carried out with a photolithographic method. Atomic force microscopy measurements showed that the polymer was formed on the disks in response to ultraviolet irradiation. Protein adsorption was reduced on the polymer‐immobilized regions, and in those regions, spreading and adhesion of mammalian cells were reduced in comparison with that in nonimmobilized regions. In conclusion, a novel histidine‐containing polymer was photoreactively immobilized on a conventional polymer surface, and it had reduced interaction with proteins and cells. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009 |
| |
| Keywords: | biocompatibility biological applications of polymers biomaterials lithography surfaces |
|
|
