Exploration of ionic modification in dual‐layer hollow fiber membranes for long‐term high‐performance protein separation |
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| Authors: | Yi Li Sim Chuan Soh Tai‐Shung Chung Sui Yung Chan |
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| Affiliation: | 1. Dept. of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 119260, Singapore;2. Dept. of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 119260, Singapore, and Singapore‐MIT Alliance, National University of Singapore, Singapore 119260, Singapore;3. Dept. of Pharmacy, National University of Singapore, Singapore 119260, Singapore |
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| Abstract: | Two types of ionic modification approaches (i.e., sulfonation and triethylamination) were applied with the aid of dual‐layer hollow fiber technology in this work to fine tune the pore size and pore size distribution, introduce the electrostatic interaction, and reduce membrane fouling for long‐term high‐performance protein separation. A binary protein mixture comprising bovine serum albumin (BSA) and hemoglobin (Hb) was separated in this work. The sulfonated fiber exhibits an improved BSA/Hb separation factor at pH = 6.8 compared with as‐spun fibers but at the expense of BSA sieving coefficient. On the other hand, the triethylaminated fiber reveals the best and most durable separation performance at pH = 4.8. Its BSA/Hb separation factor is maintained above 80 for 4 days and maximum BSA sieving coefficient reaches 33%. Therefore, this study documents that an intelligent combination of both size‐exclusion and electrostatic interaction can synergistically enhance protein separation performance in both purity and concentration. © 2008 American Institute of Chemical Engineers AIChE J, 2009 |
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| Keywords: | dual‐layer hollow fiber membranes ionic modification protein separation size‐exclusion electrostatic interaction |
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