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Probing the Role of Backbone Hydrogen Bonding in a Critical β Sheet of the Extracellular Domain of a Cys‐Loop Receptor |
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| Authors: | Kristin R. Gleitsman Henry A. Lester Dr. Dennis A. Dougherty Dr. |
| Affiliation: | 1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91106 (USA), Fax: (+1)?626‐564‐9297;2. Division of Biology, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91106 (USA) |
| Abstract: | Probing the sheet : The network of hydrogen bonds formed in the outer β sheet of the nicotinic acetylcholine receptor (nAChR; see figure) is fairly robust and tolerates single amide‐to‐ester mutations throughout. However, eliminating two proximal hydrogen bonds completely destroys receptor function; this adds further support to gating models that ascribe important roles to these β strands of the nAChR extracellular domain.  |
| Keywords: | allosterism backbone esters ion channels mutagenesis receptors |