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A Targeted Releasable Affinity Probe (TRAP) for In Vivo Photocrosslinking |
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| Authors: | Ping Yan Dr Ting Wang Dr Gregory J Newton Tatyana V Knyushko Dr Yijia Xiong Dr Diana J Bigelow Dr Thomas C Squier Dr M Uljana Mayer Dr |
| Affiliation: | 1. Novozymes, Inc., 1445 Drew Ave, Davis, CA 95618 (USA);2. Cell Biology and Biochemistry Group, Pacific Northwest National Laboratory, 999 Battelle Boulevard,Stop P7‐54, Richland, WA 99354 (USA), Fax: (+1)?509‐376‐6767;3. The University of Tokyo, Komaba Open Laboratory, Room 305, 4‐6‐1, Komaba, Meguro‐ku, Tokyo, 153‐8904 (Japan);4. Harris IT Services, NIH/NCI, 9030 Old Georgetown Road, 82, Bethesda, MD 20814 (USA) |
| Abstract: | A protein TRAP : The in vivo photocrosslinking of TRAP after its intracellular targeting to a binding sequence on the bait protein stabilizes protein interactions. Because the crosslinker is releasable, simple mass spectrometry can be used to identify the protein binding sites after purification.  |
| Keywords: | arsenic bioorganic chemistry fluorescent probes mass spectrometry photoaffinity labeling |