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Microsphere‐Mediated Protein Delivery into Cells |
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| Authors: | Rosario M Sanchez‐Martin Dr Lois Alexander Mathilde Muzerelle Dr Juan M Cardenas‐Maestre Anestis Tsakiridis Joshua M Brickman Mark Bradley Prof |
| Affiliation: | 1. School of Chemistry, Chemical Biology Section, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh EH9 3JJ (UK), Fax: (+44)?131‐6506453;2. Present address: Merck Serono International S.A. 9 Chemin des Mines, Case postale 54, 1211 Geneva 20 (Switzerland);3. MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JQ (UK), Fax: (+44)?131‐6506453 |
| Abstract: | Delivering the goods : By coupling proteins to varyingly sized polymeric microspheres, it is possible to deliver them to cells in an easy and effective way. For this study a fluorescent protein (EGFP) and a functional enzyme (β‐galactosidase) were coupled to these particles. Evaluation of the cellular uptake after “beadfection” shows that the functionality and activity of these proteins were not adversely affected through coupling to the carrier system; this shows that their functional structure is retained.  |
| Keywords: | carrier system fluorescent probes intracellular protein delivery microspheres nanoparticles |