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Synthesis of a Fluorogenic Analogue of Sphingosine‐1‐Phosphate and Its Use to Determine Sphingosine‐1‐Phosphate Lyase Activity |
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| Authors: | Carmen Bedia Dr Luz Camacho Josefina Casas Dr José Luis Abad Dr Antonio Delgado Prof Paul P Van Veldhoven Prof Gemma Fabriàs Dr |
| Affiliation: | 1. Department of Biomedicinal Chemistry, Institut de Química Avan?ada de Catalunya, CSIC, Jordi Girona 18, 08034 Barcelona (Spain), Fax: (+34?)93‐2045904;2. Unit of Medicinal Chemistry (CSIC Associated Unit), University of Barcelona, Faculty of Pharmacy, Avda. Joan XXIII s/n;3. 08028 Barcelona (Spain);4. Departement Moleculaire Celbiologie, LIPIT, Katholieke Universiteit Leuven, Herestraat 49 box 601, 3000 Leuven (Belgium) |
| Abstract: | Illuminating an ER enzyme : We report on the design and synthesis of a fluorogenic chemical sensor ( 1 ) to measure sphingosine‐1‐phosphate lyase activity in high‐throughput screening formats, as well as its validation using lyase knockout (Sgpl1?/?) cells.  |
| Keywords: | enzymes fluorescence high‐throughput screening retro reactions sphingolipids |