| 乳酸菌两组分食品级选择标记复制子缺失质粒的构建 |
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| 引用本文： | 徐波,曹郁生,周燕琴. 乳酸菌两组分食品级选择标记复制子缺失质粒的构建[J]. 食品科学, 2007, 28(7): 315-319 |
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| 作者姓名： | 徐波 曹郁生 周燕琴 |
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| 作者单位： | 江西农业大学生物科学与工程学院； 食品科学教育部重点实验室 南昌大学中德联合研究院； 食品科学教育部重点实验室； 南昌大学中德联合研究院； 江西南昌330045； 江西南昌330047； |
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| 基金项目： | 江西农业大学校科研和教改项目 |
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| 摘 要： | 乳酸菌NICE系统的两组分食品级选择标记具有显性和互补选择标记的优点。含红霉素抗性选择标记的复制子缺失的伴生型质粒是依赖复制子完全且无选择标记的食品级克隆载体而复制的。构建的伴生型质粒的Emr和rep分别来源于pMG36e和pRAF800,经酶切、缺刻、补平和连接得到只能在红霉素选择压力下与θ型复制子的克隆载体共存的复制子缺失伴生型质粒。
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| 关 键 词： | 乳酸菌 伴生型质粒 两组分选择标记 |
| 文章编号： | 1002-6630（2007）07-0315-05 |
| 修稿时间： | 2007-06-03 |
Construction of A Replicon-deficient Plasmid of Two-component Food-grade Selectable Marker for Lactococcus lactis |
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| XU Bo,CAO Yu-sheng,ZHOU Yan-qin. Construction of A Replicon-deficient Plasmid of Two-component Food-grade Selectable Marker for Lactococcus lactis[J]. Food Science, 2007, 28(7): 315-319 |
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| Authors: | XU Bo CAO Yu-sheng ZHOU Yan-qin |
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| Affiliation: | 1.College of Biology Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China;2.Key Laboratory of Food Science, Ministry of Education, Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China |
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| Abstract: | The two-component food-grade selectable marker for Lactococcus. lactis NICE system (the nisin-controlled expression system) has the advantages of dominant and complementation markers. One component is a replicon-deficificient companion plasmid, which carries an erythromycin resistance gene (Emr) as a selectable marker and it's replication is depended on the other food-grade cloning vector which carries the functional replicon, is entirely made up of L.lactis DNA and has no selection marker. The pMG36e and pRAF800 are the sources of Emr and rep for the pEmr:rep. The rep B-deficient companion plasmid can be constructed by enzyme digestion, deficiency and ligation and coexisting with the theta replicon only. |
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| Keywords: | Lactococcus lactis companion plasmid two-component selection marker |
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