Identification of alpha 1-acid glycoprotein (orosomucoid) in human synovial fluid by capillary electrophoresis |
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Authors: | A Rice J Grimshaw J Trocha-Grimshaw P McCarron GB Wisdom |
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Affiliation: | School of Biology and Biochemistry, Queen's University of Belfast, UK. |
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Abstract: | Capillary electrophoresis of human synovial fluid in a phosphate borate run buffer containing sodium dodecyl sulphate separates a hydrophilic glycoprotein, hyaluronan and a number of low-molecular-mass components. The hydrophilic glycoprotein is identified as alpha 1-acid glycoprotein (AGP), orosomucoid, by co-injection methods with human AGP and by reaction with neuraminidase which released N-acetylneuraminic acid. Finally, a sample of the glycoprotein was isolated by micropreparative capillary electrophoresis, examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis methods and shown to give a positive reaction with AGP antibodies. The peak due to AGP in the capillary electrophoresis is broad and gives evidence for the presence of glycoforms. |
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