高效液相色谱法测定愈创木酚甘油醚、福尔可定和马来酸氯苯那敏含量

采用高效液相色谱法同时测定愈创木酚甘油醚、福尔可定和马来酸氯苯那敏的含量.色谱柱为C18 (4.6mm×250 mm,5 μm),流动相为0.05 mol·L-1磷酸二氢钾溶液(每1000 mL加庚烷磺酸钠4.0g、三乙胺1.0 mL,用磷酸调pH值至2.5)—乙腈—甲醇(50∶ 20∶ 30),流速为1.0 mL·m...

Determination of Content of Guaiphenesin,Pholcodine and Chlorphenamine Maleate by HPLC

A method for determination of content of guaiphenesin,pholcodine and chlorphenamine maleate by HPLC at the same time was established.C18 Column(4.6 mm×250 mm,5 μm) was used with a mobile phase of 0.05 mol·L-1 monopotassium phosphate solution(containing 4.0 g·L-1 heptane sulfonic acid sodium and 1.0 mL·L-1 triethylamine,with pH value adjusted to 2.5 by phosphoric acid)-acetonitrile-methanol(50∶20∶30)and the flow rate was 1.0 mL·min-1.Guaiphenesin was detected at 224 nm,pholcodine and chlorphenamine maleate were detected at 214 nm.The results showed that guaiphenesin,pholcodine and chlorphenamine maleate presented a good linear relationship between amount and peak area(R=0.9999,n=5) within the range of 16.04～80.20 μg·mL-1,7.96～39.80 μg·mL-1 and 8.16～40.80 μg·mL-1,the average recovery(n=9) was 99.2%,99.0% and 99.5%,respectively.The method is simple and feasible.It is suitable for the determination of content of guaiphenesin,pholcodine and chlorphenamine maleate at the same time.