火焰原子吸收光谱法测定人血白蛋白中的钾和钠

建立了火焰原子吸收光谱法测定人血白蛋白中钾和钠的分析方法。样品中加入CsCl消除测定K、Na时的电离干扰，同时克服了试液中大量Na对测定K的干扰。由于样品中Na含量比较高，在测定时选用次灵敏线330．3nm，可减少稀释倍数、节省试剂。对样品进行的消化处理和非消化处理对比试验表明：两种处理法测定结果无明显差异，但非消化处理的测定结果精密度较高（即RSD小），手续简便，只要将样品稀释后，加入CsCl，定容后即可进行测定。K、Na的检出限分别为0．0778mg／L，1．212mg／L；特征浓度分别为0．033mg／L（1％吸收），2．864mg／L（1％吸收）；方法的精密度（RSD，n=8）分别为1．58％～1．63％和1．10％～1．16％，样品加标回收率分别为98．6％～102．4％，98．0％～101．1％。

Flame Atomic Absorption Spectrometry Determination of Kalium and Sodium in Human Serum Albumin

The analysis is proposed for the determination of kalium and sodium in human serum albumin by flame atomic absorption spectrometry.CsCl is added to eliminate the ionization interference for the determination of kalium and sodium.A large amount of sodium is eliminated for the determination of kalium.The absorbing line of 330.3 nm is selected to determine sodium because of the high content of sodium in the samples.Dilution multiple is decreased and reagent is saved for the determination.The contrast examination is carried out under assimilation treatment and non-assimilation treatment condition.The examination results indicate that there is not a distinct difference in the analysis under the two conditions.The precision of the analysis result is better and the relative standard deviation(RSD) is smaller under the non-assimilation treatment condition. The samples are diluted to volume and CsCl is added. The detection limits are 0.077 8 mg/L for K and 1.212 mg/L for Na. The character concentrations are 0.033 mg/L(1% absorption) for K and 2.864 mg/L(1% absorption) for Na with precision (RSD,n=8) 1.58%-1.63% for K, 1.10%-1.16% for Na. The recoveries of the method are 98.6%-102.4% for K and 98.0%-101.6% for Na.