| 微波酶解协同提取猴头菌丝体多糖工艺 |
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| 引用本文： | 苗晓燕,陈萍,崔珏,张筱梅,孙文敬.微波酶解协同提取猴头菌丝体多糖工艺[J].食品科学,2012,33(2):94-97. |
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| 作者姓名： | 苗晓燕 陈萍 崔珏 张筱梅 孙文敬 |
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| 作者单位： | 1.保定学院生化系 2.徐州工程学院食品学院 3.江苏大学食品与生物工程学院 |
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| 基金项目： | 河北省教育厅资助项目(Z2008401) |
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| 摘 要： | 在相同条件下利用复合酶酶解处理猴头菌丝体，即加入5mL 1%复合酶(果胶酶:纤维素酶质量比为1:1)、调pH4.2、50℃水浴酶解30min。在此基础上，采用单因素试验研究微波功率、微波时间及料液比对猴头菌丝体多糖得率的影响。再利用正交试验设计，确定微波酶解协同提取猴头菌丝体多糖的最佳工艺为微波功率500W、微波时间3min、料液比1:50(g/mL)，此条件下提取率为8.01%。
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| 关 键 词： | 猴头菌丝体 多糖 微波酶解协同 |
Microwave-Assisted Enzymatic Extraction of Polysaccharides from Cultured Mycelia of Hericium erinaceus |
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| MIAO Xiao-yan,CHEN Ping,CUI Jue,ZHANG Xiao-mei,SUN Wen-jing.Microwave-Assisted Enzymatic Extraction of Polysaccharides from Cultured Mycelia of Hericium erinaceus[J].Food Science,2012,33(2):94-97. |
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| Authors: | MIAO Xiao-yan CHEN Ping CUI Jue ZHANG Xiao-mei SUN Wen-jing |
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| Affiliation: | 1. Department of Biochemistry, Baoding University, Baoding 071000, China；
2. College of Food Science and Biotechnology, Xuzhou Institute of Technology, Xuzhou 221008, China；
3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China |
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| Abstract: | In this work,the cultured mycelia of Hericium erinaceus were hydrolyzed by 5 mL of a 1% mixture of pectinase and cellulase at a mass ratio of 1:1 in a 50 ℃ water bath for 30 min.In order to increase polysaccharide yield,microwave was used to intensify the hydrolysis of Hericium erinaceus mycelia,and operating parameters such as microwave power,treatment time and material-to-liquid ratio were optimized using one-factor-at-a-time combined with orthogonal array design.The optimal microwave treatment conditions were found to be 500 W,3 min,and 1:50(g/mL).Under these conditions,the extraction rate of polysaccharides was 8.01%. |
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| Keywords: | Hericium erinaceus mycelium polysaccharides microwave-assisted enzymatic hydrolysis |
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