X-5大孔树脂对银杏叶总黄酮吸附性能研究

用微波辅助法得到黄酮粗提液，利用分光光度法测定样品中黄酮含量，考察了吸附剂用量、粗提液浓度、pH值、吸附时间等对吸附结果的影响。静态吸附的最佳条件：树脂质量与提取液体积比1：30、pH2．5—4．0、提取液中黄酮含量为0．22—0．32mg／mL、恒温振荡70min，X-5树脂对银杏黄酮静态吸附率达70．54％。动态吸附的最佳条件为：提取液上样浓度为0．30—0．43mg／mL、pH2．8～3．5、以0．8mL／min的流速通过径高比为1：9．5的层析柱，X-5树脂对银杏黄酮动态吸附率达45．32％。结果表明，X-5树脂可较好吸附银杏叶总黄酮。

Study on Adsorption Behaviors of Flavonoid from Ginkgo Biloba Leaves with X-5 Macroporous Resin

Flavonoid extraction was obtained by assistance of microwave, and the content of flavonoid was determined by spectrophotometer. The effects of dosage of absorbent, concentration of flavonoid extract, value of pH, and absorbing time on the adsorption were examined. The adsorption rate of flavonoid was 70.54% for static adsorption under the conditions as follows： the ratio of resin mass to extract volume 1 ： 30, pH 2.5 - 4.0, flavonoid content in the extract 0.22 -0.32 mg/mL, shaking time 70 minutes at the constant temperature. The adsorption rate of flavonoid was 45.32% for dynamic adsorption under the conditions as follows： flavonoid concentration in the liquid 0.30 - 0.43 mg/mL, pH 2.8 - 3.5, ratio of column diameter to height 1 ： 9.5, the velocity of fluid 0.8 mL/min. The result showed X - 5 resin had good adsorption for flavonoid in ginkgo biloba leaves.