| pH值反馈补糖策略在生物合成丁二酸过程中的应用 |
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| 引用本文： | 曹兰彬,马江锋,陈韶辉,刘经伟,方晓江,姜岷.pH值反馈补糖策略在生物合成丁二酸过程中的应用[J].中国酿造,2011(12). |
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| 作者姓名： | 曹兰彬 马江锋 陈韶辉 刘经伟 方晓江 姜岷 |
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| 作者单位： | 1. 中国石化扬子石油化工有限公司,江苏南京,210048
2. 南京工业大学生物与制药工程学院,江苏南京,210009 |
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| 基金项目： | 国家自然科学基金(21076105) |
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| 摘 要： | 该研究通过确定大肠杆菌在产酸阶段葡萄糖的消耗与酸中和剂碳酸钠间的定量关系,建立了pH值反馈控制的补糖策略,能够维持发酵液中葡萄糖浓度在稳定水平.与分批发酵相比,采用pH值反馈补糖且维持葡萄糖浓度在较低的水平对丁二酸的积累是有利的.当采用pH值反馈补糖并控制葡萄糖浓度在109/L时,丁二酸最终浓度达到57.6g/L,生产效率达到1.15g/(L·h).
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| 关 键 词： | 丁二酸 大肠杆菌 pH值反馈 |
Application of sugar addition by pH-feedback strategy on biosynthesis of succinic acid |
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| CAO Lanbin
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MA Jiangfeng
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CHEN Shaohui
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LIU jingwei
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FANG Xiaojiang
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JIANG Min.Application of sugar addition by pH-feedback strategy on biosynthesis of succinic acid[J].China Brewing,2011(12). |
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| Authors: | CAO Lanbin MA Jiangfeng CHEN Shaohui LIU jingwei FANG Xiaojiang JIANG Min |
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| Affiliation: | CAO Lanbin1,MA Jiangfeng1,CHEN Shaohui1,LIU jingwei1,FANG Xiaojiang1,JIANG Min2(1.Sinopec Yangzi Petrochemical Co.,Ltd.,Nanjing 210048,China,2.College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | A pH-feedback strategy was established by investigation of consumption ratios of glucose and Na2CO3,which was used for neutralization of acid during the succinic acid production stage by Escherichia coli.Application of the strategy could maintain glucose concentration at a constant level.Compared with the batch fermentation,it was favourable for succinic acid accumulation when pH-feedback fermentation was adopted and glucose concentration maintained at a low level.In the case of controlling glucose concentr... |
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| Keywords: | succinic acid Escherichia coli pH-feedback |
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