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| 三波长紫外分光法测定覆盆子黄酮的研究 | |
| 引用本文： | 孙金旭,朱会霞,肖冬光,齐善厚,张家瑞. 三波长紫外分光法测定覆盆子黄酮的研究[J]. 中国酿造, 2011, 0(12) |
| 作者姓名： | 孙金旭 朱会霞 肖冬光 齐善厚 张家瑞 |
| 作者单位： | 1. 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457;衡水学院生命科学系,河北衡水053000 2. 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 3. 衡水学院生命科学系,河北衡水,053000 |
| 基金项目： | 衡水市计划项目(06003;07019-1;09058z) |
| 摘 要： | 以单波长紫外分光光度法测定覆盆子黄酮类化合物为基础,建立了三波长法定量测定覆盆子黄酮类化合物,经验证该法能有效消除杂质对定量测定的影响,平均加样回收率达到101.40％,加样回收标准偏差为2.29％,稳定性试验相对标准偏差4.90％,该方法便捷、准确,适合于覆盆子黄酮提取分离工艺探讨中快速检测黄酮含量. |
| 关 键 词： | 三波长 覆盆子 黄酮 |
Determination of raspberry flavones using three wavelength spectrophotometry |
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| SUN Jinxu , ZHU Huixia , XIAO Dongguang , QI Shanhou , ZHANG Jiarui. Determination of raspberry flavones using three wavelength spectrophotometry[J]. China Brewing, 2011, 0(12) | |
| Authors: | SUN Jinxu ZHU Huixia XIAO Dongguang QI Shanhou ZHANG Jiarui |
| Affiliation: | SUN Jinxu1,2,ZHU Huixia1,XIAO Dongguang1,QI Shanhou2,ZHANG Jiarui2(1.Ministry of Education Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China,2.Department of Biology,Hengshui College,Hengshui 053000,China) |
| Abstract: | Based on the UV scanning spectra,three-wavelength UV spectrophotometry was established to determine the content of total flavones in raspberry.This method could eliminate the interference of ethanol-soluble protein and pigments in the ethanol extract of raspberry.The average recovery rate was 101.40%,RSD was 2.29%,and RSD of stability tests was 4.90%.It was a simple,fast and accurate method. |
| Keywords: | three wavelength spectrophotometry raspberry flavones |
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